LOCUS       pLV-[hsa-miR-614]        9234 bp    DNA     circular     27-JUN-2007
SOURCE      
COMMENT     
COMMENT     This file is created by Biosettia Inc.
            http://www.biosettia.com/
FEATURES             Location/Qualifiers
     misc_feature    1807..1929
                     /label=cPPT
     CDS             7155..8015
                     /label=Amp(R)
     promoter        8216..8756
                     /label=pUC ori
     promoter        7..235
                     /label=RSV
     LTR             5760..5994
                     /label=3' LTR
     intron          526..1690
                     /label=Intron
     rep_origin      complement(6569..7024)
                     /label=f1 ori
     LTR             236..416
                     /label=5'LTR
     polyA_signal    6066..6196
                     /label=SV40 pA
     misc_feature    6197..6410
                     /label=SV40 ori
     misc_feature    5081..5677
                     /label=WPRE
     promoter        1933..2328
                     /label=human EF1a promoter
     intron          2361..3699
                     /label=intron
     CDS             3746..5071
                     /label=rPuro (RFP Puro)
     misc_RNA        3110..3489
                     /label=hsa-mir-614 precursor
BASE COUNT     2175 a      2413 c      2494 g      2152 t 
ORIGIN
        1 aagcttaatg tagtcttatg caatactctt gtagtcttgc aacatggtaa cgatgagtta 
       61 gcaacatgcc ttacaaggag agaaaaagca ccgtgcatgc cgattggtgg aagtaaggtg 
      121 gtacgatcgt gccttattag gaaggcaaca gacgggtctg acatggattg gacgaaccac 
      181 tgaattgccg cattgcagag atattgtatt taagtgccta gctcgataca taaacgggtc 
      241 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct 
      301 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga 
      361 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg 
      421 cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact 
      481 cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa 
      541 attttgacta gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg 
      601 gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat 
      661 aaattaaaac atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc 
      721 ctgttagaaa catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag 
      781 acaggatcag aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat 
      841 caaaggatag agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac 
      901 aaaagtaaga ccaccgcaca gcaagcggcc gctgatcttc agacctggag gaggagatat 
      961 gagggacaat tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg 
     1021 agtagcaccc accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat 
     1081 aggagctttg ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat 
     1141 gacgctgacg gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt 
     1201 gctgagggct attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca 
     1261 gctccaggca agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat 
     1321 ttggggttgc tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag 
     1381 taataaatct ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat 
     1441 taacaattac acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa 
     1501 gaatgaacaa gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat 
     1561 aacaaattgg ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt 
     1621 aagaatagtt tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt 
     1681 atcgtttcag acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga 
     1741 agaaggtgga gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta 
     1801 tcggttaact tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt 
     1861 agacataata gcaacagaca tacaaactaa agaattacaa aaacaaatta caaaattcaa 
     1921 aattttatcg atgcctcccc gtcaccaccc cccccaaccc gccccgaccg gagctgagag 
     1981 taattcatac aaaaggactc gcccctgcct tggggaatcc cagggaccgt cgttaaactc 
     2041 ccactaacgt agaacccaga gatcgctgcg ttcccgcccc ctcacccgcc cgctctcgtc 
     2101 atcactgagg tggagaagag catgcgtgag gctccggtgc ccgtcagtgg gcagagcgca 
     2161 catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc ggtgcctaga 
     2221 gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg 
     2281 agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg 
     2341 ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct ggcctcttta 
     2401 cgggttatgg cccttgcgtg ccttgaatta cttccacgcc cctggctgca gtacgtgatt 
     2461 cttgatcccg agcttcgggt tggaagtggg tgggagagtt cgaggccttg cgcttaagga 
     2521 gccccttcgc ctcgtgcttg agttgaggcc tggcctgggc gctggggccg ccgcgtgcga 
     2581 atctggtggc accttcgcgc ctgtctcgct gctttcgata agtctctagc catttaaaat 
     2641 ttttgatgat atcctgcgac gctttttttc tggcaagata gtcttgtaaa tgcgggccaa 
     2701 gatctgcaca ctggtatttc ggtttttggg gccgcgggcg gcgacggggc ccgtgcgtcc 
     2761 cagcgcacat gttcggcgag gcggggcctg cgagcgcggc caccgagaat cggacggggg 
     2821 tagtctcaag ctggccggcc tgctctggtg cctggcctcg cgccgccgtg tatcgccccg 
     2881 ccctgggcgg caaggctggc ccggtcggca ccagttgcgt gagcggaaag atggccgctt 
     2941 cccggccctg ctgcagggag ctcaaaatgg aggacgcggc gctcgggaga gcgggcgggt 
     3001 gagtcaccca cacaaaggaa aagggccttt ccgtcctcag ccgtcgcttc atgtgactcc 
     3061 acggagtacc gggcgccgtc caggcacctc gattagttct cgaggatccc caggcagatc 
     3121 tcgaactcca aacctcgtga tccacctacc ttggcctctg aaagtgctgg gataccgtgg 
     3181 cctctgaaag tgctgggatt acaggcatga gccaccgcgc ccggccccgt tgtttccctt 
     3241 ctaagaaacg cagtggtctc tgaagcctgc aggggcaggc cagccctgca ctgaacgcct 
     3301 gttcttgcca ggtggcagaa ggttgctgct catttgagca gtacctgtca cccctcctcc 
     3361 cactgctgcg gctgttaact catcttcagg tctcacacct tctgcacata aataagctat 
     3421 ttttaaaagt tattattttt ttactctttc ttttctttgg agagggtacc aaaggatagc 
     3481 tgttctgttg ctagctcgag cttttggagt acgtcgtctt taggttgggg ggaggggttt 
     3541 tatgcgatgg agtttcccca cactgagtgg gtggagactg aagttaggcc agcttggcac 
     3601 ttgatgtaat tctccttgga atttgccctt tttgagtttg gatcttggtt cattctcaag 
     3661 cctcagacag tggttcaaag tttttttctt ccatttcagg tgtcgtgaaa actacccctc 
     3721 tagagtcgag ctaccggtcg ccaccatggt gagcaagggc gaggaggata acatggccat 
     3781 catcaaggag ttcatgcgct tcaaggtgca catggagggc tccgtgaacg gccacgagtt 
     3841 cgagatcgag ggcgagggcg agggccgccc ctacgagggc acccagaccg ccaagctgaa 
     3901 ggtgaccaag ggtggccccc tgcccttcgc ctgggacatc ctgtcccctc agttcatgta 
     3961 cggctccaag gcctacgtga agcaccccgc cgacatcccc gactacttga agctgtcctt 
     4021 ccccgagggc ttcaagtggg agcgcgtgat gaacttcgag gacggcggcg tggtgaccgt 
     4081 gacccaggac tcctccctgc aggacggcga gttcatctac aaggtgaagc tgcgcggcac 
     4141 caacttcccc tccgacggcc ccgtaatgca gaagaagacc atgggctggg aggcctcctc 
     4201 cgagcggatg taccccgagg acggcgccct gaagggcgag atcaagcaga ggctgaagct 
     4261 gaaggacggc ggccactacg acgctgaggt caagaccacc tacaaggcca agaagcccgt 
     4321 gcagctgccc ggcgcctaca acgtcaacat caagttggac atcacctccc acaacgagga 
     4381 ctacaccatc gtggaacagt acgaacgcgc cgagggccgc cactccaccg gcggcatgga 
     4441 cgagctgtac aaggacccac cggtcgccac catgaccgag tacaagccca cggtgcgcct 
     4501 cgccacccgc gacgacgtcc ccagggccgt acgcaccctc gccgccgcgt tcgccgacta 
     4561 ccccgccacg cgccacaccg tcgatccgga ccgccacatc gagcgggtca ccgagctgca 
     4621 agaactcttc ctcacgcgcg tcgggctcga catcggcaag gtgtgggtcg cggacgacgg 
     4681 cgccgcggtg gcggtctgga ccacgccgga gagcgtcgaa gcgggggcgg tgttcgccga 
     4741 gatcggcccg cgcatggccg agttgagcgg ttcccggctg gccgcgcagc aacagatgga 
     4801 aggcctcctg gcgccgcacc ggcccaagga gcccgcgtgg ttcctggcca ccgtcggcgt 
     4861 ctcgcccgac caccagggca agggtctggg cagcgccgtc gtgctccccg gagtggaggc 
     4921 ggccgagcgc gccggggtgc ccgccttcct ggagacctcc gcgccccgca acctcccctt 
     4981 ctacgagcgg ctcggcttca ccgtcaccgc cgacgtcgag tgcccgaagg accgcgcgac 
     5041 ctggtgcatg acccgcaagc ccggtgcctg agcggccgcg tcgacaatca acctctggat 
     5101 tacaaaattt gtgaaagatt gactggtatt cttaactatg ttgctccttt tacgctatgt 
     5161 ggatacgctg ctttaatgcc tttgtatcat gctattgctt cccgtatggc tttcattttc 
     5221 tcctccttgt ataaatcctg gttgctgtct ctttatgagg agttgtggcc cgttgtcagg 
     5281 caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg gggcattgcc 
     5341 accacctgtc agctcctttc cgggactttc gctttccccc tccctattgc cacggcggaa 
     5401 ctcatcgccg cctgccttgc ccgctgctgg acaggggctc ggctgttggg cactgacaat 
     5461 tccgtggtgt tgtcggggaa gctgacgtcc tttccatggc tgctcgcctg tgttgccacc 
     5521 tggattctgc gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc agcggacctt 
     5581 ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag 
     5641 acgagtcgga tctccctttg ggccgcctcc ccgcctggaa ttcgagctcg gtacctttaa 
     5701 gaccaatgac ttacaaggca gctgtagatc ttagccactt tttaaaagaa aaggggggac 
     5761 tggaagggct aattcactcc caacgaagac aagatctgct ttttgcttgt actgggtctc 
     5821 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 
     5881 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 
     5941 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtagta 
     6001 gttcatgtca tcttattatt cagtatttat aacttgcaaa gaaatgaata tcagagagtg 
     6061 agaggaactt gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt 
     6121 tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg 
     6181 tatcttatca tgtctggctc tagctatccc gcccctaact ccgcccatcc cgcccctaac 
     6241 tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga 
     6301 ggccgaggcc gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg 
     6361 cctagggacg tacccaattc gccctatagt gagtcgtatt acgcgcgctc actggccgtc 
     6421 gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca 
     6481 catccccctt tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa 
     6541 cagttgcgca gcctgaatgg cgaatgggac gcgccctgta gcggcgcatt aagcgcggcg 
     6601 ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct 
     6661 ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat 
     6721 cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt 
     6781 gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg 
     6841 acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac 
     6901 cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta 
     6961 aaaaatgagc tgatttaaca aaaatttaac gcgaatttta acaaaatatt aacgcttaca 
     7021 atttaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa 
     7081 tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt 
     7141 gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg 
     7201 cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag 
     7261 atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg 
     7321 agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg 
     7381 gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt 
     7441 ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga 
     7501 cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac 
     7561 ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc 
     7621 atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc 
     7681 gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac 
     7741 tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag 
     7801 gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg 
     7861 gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta 
     7921 tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg 
     7981 ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata 
     8041 tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt 
     8101 ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc 
     8161 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct 
     8221 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa 
     8281 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag 
     8341 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc 
     8401 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg 
     8461 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca 
     8521 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat 
     8581 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg 
     8641 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc 
     8701 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc 
     8761 ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc 
     8821 cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg 
     8881 cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga 
     8941 gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc 
     9001 attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa 
     9061 ttaatgtgag ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc 
     9121 gtatgttgtg tggaattgtg agcggataac aatttcacac aggaaacagc tatgaccatg 
     9181 attacgccaa gcgcgcaatt aaccctcact aaagggaaca aaagctggag ctgc 
//
